detection of toxoplasma gondii antigens in sera and urine of experimentally infected mice by capture elisa

background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrificed every day from day 1 up to day 7 post infection. sera and urine of mice were tested by capture elisa. results: t.gondii antigens were detected from 3 rd and 2 nd day in serum and urine, respectively, after infection until their death on day 7. conclusion: antigenemia detection of antigens of parasite was possible in a short period of acute infection with t. gondii by capture elisa.